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pycytominer

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pycytominer is the core Python package in the Cytomining ecosystem. It provides a clean, composable API for processing single-cell morphological profiles produced by tools like CellProfiler.

Key capabilities:

  • Aggregate single-cell data to well- or treatment-level profiles
  • Annotate profiles with experimental metadata
  • Normalize features using population-level statistics
  • Select high-quality features and remove noise
  • Output analysis-ready profiles in standard formats

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Publication #

Nature Methods · 2025

Reproducible image-based profiling with Pycytominer

Serrano E, Chandrasekaran SN, Bunten D, Brewer KI, Tomkinson J, et al.

doi: 10.1038/s41592-025-02611-8