buscar
buscar scores perturbations directly on single-cell distributions using Earth Mover’s Distance, preserving heterogeneity throughout hit calling.
Problem: Population-level hit calling averages away biologically meaningful cell-to-cell variation, making heterogeneous responses and rare subpopulations invisible to standard metrics.
Key capabilities:
- Define on-target and off-target morphology signatures from reference profiles
- Score perturbation efficacy via Earth Mover’s Distance
- Assess specificity with off-target scoring to reduce false positives
- Preserve single-cell heterogeneity throughout hit calling
- Integrates directly with
Pycytominer,coSMicQC, andCytoTableworkflows